ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 110 kDa band observed in rat brain lysates (calculated MW of 105 kDa according to NP_015566.1). Recommended concentration: 2-4 μg/mL. A minor band of unknown identity was also consistently observed at 23-24 kDa . This band was strong in mouse brain lysates and faint in human brain lysates. The band was successfully blocked by incubation with the immunizing peptide.
NMDA Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NMDA Receptor antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.