ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 40 kDa band observed in human lung and H460 cell extracts (predicted MW of 40 kDa according to NP_054829). Recommended for use at 1-2 μg/mL. Immunohistochemistry: In paraffin embedded human thymus shows speckled staining of nuclei . Recommended concentration, 2.5-3.8 μg/mL.
NIPP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NIPP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.