ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 35 kDa band observed in HEK293 and Daudi lysates (calculated MW of 36.2 kDa according to NP_004776.3). Recommended concentration: 1-3 μg/mL.
NHERF2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NHERF2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.