ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 38 kDa band observed in lysates of human skin, testis and thyroid gland (calculated size of 33.3 kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8 μg/mL.
NHEJ1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NHEJ1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.