ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 55-60 kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5 kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18;94 (6) :2609-14.; PMID: 9122243). Recommended concentration: 0.5-1.5 μg/mL.
NFIL3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NFIL3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.