ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 22-23 kDa band observed in mouse brain and rat brain lysates (calculated MW of 22.4 kDa according to mouse NP_032764.1). Recommended concentration: 0.1-0.3 μg/mL. an additional very strong band of unknown identity was also consistently observed at 50 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Neurturin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Neurturin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Mouse, Rat