ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 150 kDa band in human brain lysates after 1 μg/mL antibody staining.0 kDa according to NP_055747.1. The 150 kDa band was successfully blocked by incubation with the immunizing peptide.
Neuroligin 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Neuroligin 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.