ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 300 kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3 (calculated MW of 327 kDa according to NP_056493.3). Data kindly provided by Professor John Creemers, K.U. Leuven, Belgium. Recommended concentration: 1-3 μg/mL.
Neurobeachin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Neurobeachin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse