ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 45 kDa band in human cerebellum lysates after 0.3 μg/mL antibody staining.6 kDa according to NP_037481.1 The 45 kDa band was successfully blocked by incubation with the immunizing peptide.
Neudesin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Neudesin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.