ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 100 kDa band observed in human kidney, lung and placenta lysates (calculated MW of 92.9 kDa according to NP_006394.1). Recommended concentration: 0.1-0.3 μg/mL.
NEDD9 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NEDD9 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.