ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 48 kDa band observed in lysates of human brain, kidney and testis (calculated MW of 42.8 kDa according to NP_006087.2). Recommended concentration: 0.1-0.3 μg/mL. A minor band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide.
NDRG1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NDRG1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.