ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 35 kDa band observed in nuclear HeLa lysate (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/mL. an additional band of approximately 37 kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources. Immunohistochemistry: In paraffin embedded human testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2 μg/mL.
NANOG antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NANOG antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.