ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 38 kDa band observed in human ovary lysates (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.03-0.1 μg/mL. Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody does not work in PFA-fixed frozen embryos and ESC from mouse.
NANOG antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
NANOG antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Pig