ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 45 kDa band in human brain and human liver lysates at 1 μg/mL. This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10 μg/mL.
MRIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MRIP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.