ELISA: Antibody detection limit dilution 1:128000. Western Blot: Preliminary experiments gave no signal but low background in human heart lysates at up to 1 μg/mL. However this used our routine Western Blotting protocol which we would not expect to reliably detect proteins as large as the predicted size of approximately 312 kDa according to NP_055542. Immunohistochemistry: Immunohistochemistry: In paraffin embedded human testis shows cytoplasm staining of spermatids. Recommended concentration, 5-10 μg/mL.
MLL4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MLL4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.