ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 75 kDa and 25 kDa in K562 lysate after 0.1 μg/mL antibody staining.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human placenta shows nuclear stainng in the trophoblasts . Recommended concentration, 4-8 μg/mL.
MLL2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MLL2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.