ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 13 kDa band observed in human lymph node and thymus lysates (calculated MW of 12.5 kDa according to NP_002406.1). Recommended concentration: 0.01-0.03 μg/mL.
MIF antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MIF antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.