ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 80 kDa band observed in mouse heart lysates (calculated MW of 81.3 kDa according to NP_036348.1 and 77.9 kDa according to NP_438112.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10 μg/mL.
MID2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MID2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse