ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 75 kDa band observed in rat testis lysates (calculated MW of 74.4 kDa according to human NP_062552.2 and 75.3 kDa according to rat XP_222048.4). In transfected HEK293 transiently expressing human MEPCE a band of approximately 75 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
MEPCE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MEPCE antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Rat