ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 80 kDa band observed in human peripheral blood leukocyte lysate. Recommended concentration: 1-3 μg/mL during overnight incubation.
MEFV antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MEFV antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.