ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 40 kDa band observed in human skin lysates (calculated MW of 33.9 kDa according to NP_000520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kilianova et al, Endocrinology. 2006 May;147 (5) :2325-37; PMID: 16497811). Recommended concentration: 1-3 μg/mL.
MC2R antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MC2R antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.