ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 150 kDa band observed in human placenta lysates (calculated MW of 131 kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approximately 98 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
MAN2A1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MAN2A1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.