ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 110 kDa band observed in human skeletal muscle lysates (calculated MW of 108 kDa according to NP_055572.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8 μg/mL.
MAML1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MAML1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.