ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 22 kDa band observed in human kidney lysates (predicted MW of 24 kDa according to NP_006445). Recommended for use at 0.5-2 μg/mL. Please note the appearance of this band was blocked by incubation with the immunizing peptide.
MAD4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MAD4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.