ELISA: Antibody detection limit dilution 1:64,000. Antibody has been successfully used in both Western Blot and immunohistochemistry on human brain: Alegre-Abarrategui J et al., Neuropathol Appl Neurobiol. 2007 Oct 26; PMID: 17971075. Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human hippocampus shows staining of neuronal cytoplasm. Recommended concentration, 1-2 μg/mL.
LRRK2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LRRK2 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.