ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 50 kDa and 35 kDa in human adipose lysate after 0.1 μg/mL antibody staining.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Immunohistochemistry: In paraffin embedded human kidney shows luminar membrane staining in DCT. Recommended concentration, 4-6 μg/mL.
LRP6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LRP6 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.