ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 70-75 kDa band and Approximately 30 kDa band observed in human lung lysates (predicted MWs of 92 kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approximately 50 kDa . Recommended for use at 0.5-2 μg/mL.
LMO7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LMO7 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.