ELISA: Antibody detection limit dilution 1:23,000. Western Blot: Approximately 50 kDa and 26 kDa in human adipose lysates after 0.3 μg/mL antibody staining.7 kDa according to NP_663731.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Lipin 1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Lipin 1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.