ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 25 kDa band in human duodenum lysates after 0.1 μg/mL antibody staining.2 kDa according to NP_004181.1. The25 kDa band was successfully blocked by incubation with the immunizing peptide.
LIPF antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LIPF antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.