ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 35 kDa band in lysates of human brain, placenta and prostate and of cell lines Daudi, HeLa and K562 after 0.1 μg/mL antibody staining.6 kDa according to NP_000224.2. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
LHCGR antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LHCGR antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.