ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 30-35 kDa band observed in mouse testis lysates (calculated MW of 36.6 kDa according to human NP_002292.1 and 35.9 kDa according to mouse NP_038608.1). Recommended concentration: 0.03-0.1 μg/mL.
LDHC antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LDHC antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.