ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 36 kDa band observed in human kidney lysates (calculated MW of 29.7 kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30;207 (2) :171-5; PMID: 9511759). Recommended concentration: 0.03-0.1 μg/mL.
LASP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
LASP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.