ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 70 kDa band observed in lysates of cell lines A431, HeLa and HepG2 (calculated MW of 69.8 kDa according to NP_001460.1). In transfected HEK293 transiently expressing KU70 a band of approximately 70 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3 μg/mL.
KU70 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
KU70 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.