ELISA: Antibody detection limit dilution >1/32,000. Western Blot: Approximately 55-60 kDa band observed in human testis and kidney lysates (predicted size of approximately 58 kDa according to NP_002258). Recommended for use at 0.1-0.5 μg/mL. Immunohistochemistry: In paraffin embedded human heart shows strong pixulate staining of myocardial fibres in transverse section. Recommended concentration, 5-10 μg/mL.
KPNA3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
KPNA3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse, Rat