ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 35 kDa and 60 kDa bands observed in human brain and human liver lysates at 1 on limit dilution 1:8,000. Western Blot: Approximately 35 kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 36.0 kDa according to NP_064695.2). Recommended concentration: 0.03-0.1 μg of primary. Please note that the band we observe is different from the predicted size of approximately 79 kDa according to NP_005658.
KF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
KF1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.