ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 200 kDa band observed in mouse brain lysates (calculated MW of 192 kDa according to XP_001001845). Recommended concentration: 1-3 μg/mL.
Kalirin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Kalirin antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.