ELISA: Antibody detection limit dilution 1:32,000. Approximately 170 kDa band observed in human cerebellum lysates (calculated MW of 127 kDa according to NP_000623.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. Recommended concentration: 1-3 μg/mL.
ITGAM antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ITGAM antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.