ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 110 kDa band observed in human ovary and mouse eye lysates (calculated MW of 88.1 kDa according to NP_002204.2). Recommended concentration: 0.1-0.3 μg/mL. Customer feedback: observed 110 kDa in mouse eye was confirmed and not present in knock-out mouse.
Integrin beta 5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Integrin beta 5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.
Reactivity: Human, Mouse