ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 25 kDa band observed in human adrenal gland lysates (calculated MW of 25.3 kDa according to NP_002169.1). In transfected HEK293 transiently expressing IGFBP6 a band of approximately 29 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 μg/mL.
IGFBP6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
IGFBP6 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.