ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approximately 30 kDa band observed in human liver lysates (calculated MW of 27.9 kDa according to NP_001543.2). Recommended concentration: 1-3 μg/mL.
IGFBP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
IGFBP4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.