ELISA: Antibody detection limit dilution 1:2000. Western Blot: Customer's experiments gave a band at approximately 45 kDa in lysates of transfected H1299 overexpressing IER5 but not when transfected with empty vector (calculated MW of 33.7 kDa according to NP_057629.1). Recommended concentration: 1-3 μg/mL.
IER5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
IER5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.