ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 140 kDa band observed in lysates of Daudi lysate (calculated MW of 85.9 kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274 (5287) :605-10.; PMID: 8849451). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide.
HTATSF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HTATSF1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.