ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 20-25 kDa band seen in human muscle lysate [Predicted MW of approximately 22 kDa according to NP_055180]. Recommended for use at 0.5-1 μg/mL.
HSP22 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HSP22 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.