ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 28 kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 30.7 kDa according to NP_006352.2). Recommended concentration: 0.1-0.3 μg/mL.
Homeobox B13 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Homeobox B13 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.