ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 52 kDa and 48 kDa bands observed in lysates of human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8 kDa according to NP_000448.3 and 46.6 kDa according to NP_849181.1). Recommended concentration: 0.1-0.3 μg/mL.
HNF4A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HNF4A antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.