ELISA: Antibody detection limit dilution 1:32,000. Approximately 70 kDa band observed in human brain (frontal cortex and hippocampus) lysates (calculated MW of 69.6 kDa according to NP_817084.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 25 kDa . This band was successfully blocked by incubation with the immunizing peptide.
HAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HAP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.