ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 33 kDa band observed in human heart, muscle and kidney lysates (calculated MW of 34.3 kDa according to NP_005318.2). In transfected HEK293 transiently expressing HADH a band of approximately 38 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/mL.
HADH antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HADH antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.