ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 35 kDa band observed in human placenta lysates (calculated MW of 34.5 kDa according to NP_057399.1). Recommended concentration: 0.2-0.6 μg/mL. an additional band of unknown identity was also consistently observed at approximately46 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules. Recommended concentration, 5-10 μg/mL.
GULP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GULP1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.