ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 37 kDa band in lysates of cell lines HepG2 and HeLa after 0.1 μg/mL antibody staining. The 37 kDa band was successfully blocked by incubation with the immunizing peptide.
GPX6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GPX6 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.