ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 19 kDa band observed in human kidney and testis lysates and in HEK293 lysate (calculated MW of 22.1 kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3 μg/mL.
GPX4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
GPX4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% BSA.
Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles.